DL-Dithiothreitol

What is DL-Dithiothreitol? It is a chemical compound primarily used as a reducing agent in various scientific and biochemical applications. Its main function is to reduce disulphide bonds in proteins and other molecules, preventing unwanted oxidation and maintaining thiol groups in their reduced state. 

Consistent Quality: We provide only high-quality DL-Dithiothreitol, ensuring reliable and consistent performance for all applications.

Shipped from Manchester, UK Facility: Sourced and packaged in non-toxic, chemical-resistant containers to ensure safe handling and preserve product quality during transport.

Frequent Uses

Reducing Protein Disulfide Bonds
DTT is a key reagent in sample buffers for SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis), where it reduces disulphide bonds in proteins, facilitating their separation based on size. 

Maintaining Reduced Thiols
Used to prevent the oxidation of thiol (-SH) groups in proteins and other molecules, which is crucial for maintaining their activity and preventing unwanted aggregation. 

In-solution Protein Digestion
Used in proteomics for digesting proteins in solution prior to mass spectrometry analysis. 

Enzyme Stabilisation
Can act as an enzyme stabiliser by preventing the modification of DNA. 

• SDS-PAGE
• Mass spectrometry analysis
• DNA research

Assay: 99.0 - 102.%
MF: C₄H₁₀O₂S₂
MW: 154.25g/mol
FP: 113°C
Description: White free flowing powder, hydroscopic
Solubility: Freely soluble in water, methanol & methylene chloride
Clarity (Water): 5% w/v solution in water is clear & colourless
Clarity (Sodium acetate): Clear & colourless  

Identification:-
-IR Spectrum: IR spectrum of sample exhibits maxima at the same wavelengths as of a similar preparation of
   reference compound
-TLC: IR value of the sample is comparable with that of the standard

Related substance:-
-TLC: Single individual impurity NMT 0.5%
Total impurities not more than 2.0%
-UV Spectroscopy: Context of oxidised DTT (Dihydroxydithiane) NMT 0.4%

UV Absorbance:-
500nm: Absorbance of 0.1M solution at 500nm NMT 0.050
280nm: Absorbance of 0.1M solution at 280nm NMT 0.100
pH/Acidity: pH of 0.1M solution in water is between 4.0 & 6.0

Assay (by iodimetry): NTL 99%
Loss on drying: < 0.5%
Purity by GC: > 98%
Heavy metals as Lead (Pb): NMT 1mg per kg
Sulphate: NMT 50 mg per kg
Melting range: 42—44˚C

Cas Number: 3483-12-3

Einecs Number: 222-468-7

HS Code / Commodity Code: 2930909899

Hazard Phrases: H302,H315,H319

Prec Phrases: P301+P312,P330,P305+P351+P338