Urea Broth Base

A modification of Christensen's medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other Enterobacteriaceae including urease-producing Salmonella spp. and Shigella spp.

Unlike Christensen's medium when used for the later purpose, it is not necessary to increase the incubation time. The peptone provides the required nitrogen, carbon and vitamins.

Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator.

Used to detect rapid urease activity of Proteus spp.

Additional Infomation

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale Orange

Dehydrated PH: 6.8 ± 0.2

Prepared PH: 6.8 ± 0.2

Recommended Incubation: Aerobically at 37°C ± 1°C for 4-6 hours