Tryptone Soya Broth

A general-purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms.

Tryptone and soy peptone are the nitrogen and vitamin sources in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance.

Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB.

TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).

Nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Pale Straw

Prepared Medium Colour: Pale Straw

Dehydrated PH: 7.3 ± 0.2

Prepared PH: 7.3 ± 0.2

Recommended Incubation: As required by organisms according to the methodology being followed

g/L
Tryptone: 17.0

Soy peptone: 3.0

Sodium chloride: 5.0

Glucose: 2.5

Di potassium hydrogen phosphate: 2.5

Total 30.0

*Adjusted/supplemented as required to meet performance requirements

 

g/L
Tryptone	17.0
Soy peptone	3.0
Sodium chloride	5.0
Glucose	2.5
Di potassium hydrogen phosphate	2.5
Total	30.0
*Adjusted/supplemented as required to meet performance requirements