T.C.B.S Medium

Code: KM0008-500G

Revert

Variation ID	Product Code	Pack Size	List Price	Qty
18062	KM0008-500G	500g	£82.20

Revert

Pack: 500g

£82.20

Thiosulphate citrate bile salts sucrose (TCBS) agar is a selective medium used in microbiology laboratories to isolate Vibrio spp.

The formulation was developed by Kobayashi et al. which was modified from the Nakanishis formulation. The yeast extract and peptone are the source of the required nitrogen, carbon and vitamins.

TCBS agar contains high concentrations of sodium thiosulphate, sodium citrate and oxbile to inhibit the growth of Gram-positive organisms and suppress coliforms. Sucrose is included as a fermentable carbohydrate for the metabolism of Vibrio spp.

Sodium chloride maintains the osmotic balance in the medium. Sodium thiosulphate also serves as a sulphur source and, in combination with ferric citrate, detects hydrogen sulphide production. Thymol blue and bromothymol blue are included as indicators of pH changes.

Selective medium used in microbiology laboratories to isolate Vibrio spp.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Dark Green

Dehydrated PH: 8.6 ± 0.2

Prepared PH: 8.6 ± 0.2

Recommended Incubation: Aerobically at 37°C ± 1°C for 18-24 hours

Formula* g/L

Yeast extract: 5.0