Sheep Blood Agar Base

Sheep Blood Agar Base has been developed to provide a nutrient-rich medium compatible with sheep blood for the cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions.

Alternative culture mediums, such as Blood agar base No. 2, can result in mixed haemolytic reactions for some Streptococcus spp.

This is thought to occur due to the trace amounts of fermentable carbohydrates in yeast extract and the physiological difference between sheep and horse blood. The tryptone, peptone, and yeast extract provide the required nitrogen, carbon and vitamins in the medium. Sodium chloride maintains the osmotic balance.

Cultivation of many bacteria, especially fastidious Streptococcus spp., without affecting haemolytic reactions.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Red

Dehydrated PH: 7.3 ± 0.2

Prepared PH: 7.3 ± 0.2

Recommended Incubation: Aerobically at 30-35°C for 18-24 hours

Formula g/L:

Tryptone: 14.0

Peptone: 4.5

Yeast Extract: 4.5

Sodium Chloride: 5.0

agar: 12.0

Total: 40