Selenite Cystine Broth

Selenite cystine broth is a modification of selenite F broth and is for the selective enrichment of Salmonellae spp. from clinical, food and environmental specimens.

The peptone acts as a nitrogen, carbon and vitamin source. Lactose is a fermentable carbohydrate and sodium phosphate is a buffer. L-cystine is used to enhance the recovery of Salmonellae spp. in low numbers.

The medium is made selective by the addition of sodium biselenite (KM8021). Following overnight incubation subculture(s) are usually made onto one or more of the many selective enteric solid media.

selective enrichment of Salmonellae spp. from clinical, food and environmental specimens.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance#: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Clear to Pale Orange

Dehydrated PH: 7.1 ± 0.2

Prepared PH: 7.1 ± 0.2

Recommended Incubation: Aerobically at 35-37°C for 18-24 hours

Formula g/L

Peptone: 5.0

Lactose: 4.0

Sodium Phosphate: 10.0

L-Cystine: 0.01

Total: 19.0