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Perfringens Agar Base
Code: KM0147-500G
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| Variation ID | Product Code | Pack Size | List Price | Qty | |
|---|---|---|---|---|---|
| KM0147-500G | 500g | £88.20 |
Revert
Pack: 500g
£88.20
Perfringens Agar Base is a base medium that allows the enumeration of Clostridium perfringens from food samples. As a base medium, Perfringens Agar Base can have several supplementary and selective agents added to increase selectivity.
The addition of Eye Yolk Emulsion (BM0140) allows the detection of lecithinase activity as precipitates are formed by C. perfringens. The addition of D-cycloserine (LS0023) can be used to inhibit other facultative anaerobes, as used in Tryptose-Sulphite-Cycloserine (TSC) agar. (1&2)Alternately, kanamycin and polymyxin B (LS0025) can be used to inhibit other coliforms generally found in food samples, as used in Shahidi-Ferguson Perfringens (SFP) agar. (3)The tryptose and soy peptone provide the required nitrogen, carbon and minerals. Yeast extract provides the essential vitamins, including the vitamin B group, needed for growth.
The ferric ammonium citrate and sodium metabisulphite allow the reduction of sulphites to hydrogen sulphide by C. perfringens, which produces black colonies.References(1) ISO- 7937:2004. Microbiology of food and animal feeding stuff- Horizontal method for the enumeration of Clostridium perfringens Colony count technique(2) Harmon, S.M., Kauttar, D.A. and Peeler, J.T. 1971.
Improved Medium for Enumeration of Clostridium perfringens. Appl. Microbiol. 22:688-692.(3) Shahidi S. A. and Ferguson A. R. 1971 New Quantitative, Qualitative, and Confirmatory Media for Rapid Analysis of Food for Clostridium perfringens. Appl. Microbiol. 21:500-506Enquire About Product
Perfringens Agar Base is a base medium that allows the enumeration of Clostridium perfringens from food samples.
Shelf Life (days): 1095 days
Storage: Once opened keep the powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated Medium Appearance: Fine Powder
Dehydrated Medium Colour: Beige
Dehydrated PH: 7.6 ± 0.2
Prepared Medium Appearance: Firm Gel
Prepared Medium Colour: Dark Straw
Prepared PH: 7.6 ± 0.2
Formula g/L
Tryptose: 15.0
Soy Peptone: 5.0
Yeast Extract: 5.0
Sodium Metabisulphite: 1.0
Ferric Ammonium Citrate: 1.0
Agar: 19.0
Total: 46.0