Perfringens Agar Base

Code: KM0147-500G

Variation ID		Product Code	Pack Size	List Price	Qty
18037	photo_camera	KM0147-500G	500g KM0147-500G	£88.20

Pack: 500g

£88.20

Perfringens Agar Base is a base medium that allows the enumeration of Clostridium perfringens from food samples. As a base medium, Perfringens Agar Base can have several supplementary and selective agents added to increase selectivity.

The addition of Eye Yolk Emulsion (BM0140) allows the detection of lecithinase activity as precipitates are formed by C. perfringens. The addition of D-cycloserine (LS0023) can be used to inhibit other facultative anaerobes, as used in Tryptose-Sulphite-Cycloserine (TSC) agar. (1&2)Alternately, kanamycin and polymyxin B (LS0025) can be used to inhibit other coliforms generally found in food samples, as used in Shahidi-Ferguson Perfringens (SFP) agar. (3)The tryptose and soy peptone provide the required nitrogen, carbon and minerals. Yeast extract provides the essential vitamins, including the vitamin B group, needed for growth.

The ferric ammonium citrate and sodium metabisulphite allow the reduction of sulphites to hydrogen sulphide by C. perfringens, which produces black colonies.References(1) ISO- 7937:2004. Microbiology of food and animal feeding stuff- Horizontal method for the enumeration of Clostridium perfringens Colony count technique(2) Harmon, S.M., Kauttar, D.A. and Peeler, J.T. 1971.

Improved Medium for Enumeration of Clostridium perfringens. Appl. Microbiol. 22:688-692.(3) Shahidi S. A. and Ferguson A. R. 1971 New Quantitative, Qualitative, and Confirmatory Media for Rapid Analysis of Food for Clostridium perfringens. Appl. Microbiol. 21:500-506Enquire About Product

Perfringens Agar Base is a base medium that allows the enumeration of Clostridium perfringens from food samples.

Shelf Life (days): 1095 days

Storage: Once opened keep the powdered medium closed to avoid hydration and use before the expiry date on the label.

Dehydrated Medium Appearance: Fine Powder

Dehydrated Medium Colour: Beige

Dehydrated PH: 7.6 ± 0.2

Prepared Medium Appearance: Firm Gel

Prepared Medium Colour: Dark Straw

Prepared PH: 7.6 ± 0.2

Formula g/L

Tryptose: 15.0

Soy Peptone: 5.0