Mannitol Salt Agar

Code: KM0031-500G

Variation ID		Product Code	Pack Size	List Price	Qty
18023	photo_camera	KM0031-500G	500g KM0031-500G	£62.40

Pack: 500g

£62.40

Mannitol Salt Agar is a selective medium for the isolation of pathogenic staphylococci. The medium conforms to the requirements of the Harmonised USP/EP/JP. Chapman showed that adding a high level of sodium chloride to Phenol Red Mannitol Agar allowed for the recovery of pathogenic staphylococci.

Pathogenic staphylococci produced yellow colonies whereas non-pathogenic staphylococci produced small red colonies. Pancreatic digestion of casein, peptic digestion of animal tissue and beef extract provide the required nitrogen, carbon and vitamins.

The high level of sodium chloride inhibits most organisms other than staphylococci. Mannitol is a carbohydrate that is fermented by coagulase-positive staphylococci. Phenol red is the pH indicator.

Selective medium for the isolation of pathogenic staphylococci.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Pink

Dehydrated PH: 7.4 ± 0.2

Prepared PH: 7.4 ± 0.2

Recommended Incubation: Aerobically at 30-35°C for 18-72 hours

Formula g/L

Pancreatic digest of casein: 5.0