MacConkey Agar with Salt

This is a selective medium for the isolation and differentiation of bile-tolerant Gram-negative (enteric) and Gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology.

This medium is recommended by the World Health Organisation and the Department of Health for the bacteriological examination of water.

It has the disadvantage that many strains of Proteus spp. will spread on it and for this reason MacConkey Agar without salt and crystal violet may be preferred (KM0011).

selective medium for the isolation and differentiation of bile-tolerant Gram-negative (enteric) and Gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale pink/red

Dehydrated PH: 7.4 ± 0.2

Prepared PH: 7.4 ± 0.2

Recommended Incubation: Aerobically at 37°C for 18-24 hour

Formula g/L

Peptone: 20.0

Lactose: 10.0

Bile Salts: 5.0

Sodium Chloride: 5.0

Neutral Red: 0.05

Agar 12.5

Total: 52