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MacConkey Agar No.3 Group Picture

MacConkey Agar No.3

Code: KM0027-500G
  Pack Size List Price Qty  
photo_camera 500g
KM0027-500G
£78.15
Pack: 500g

£78.15

MacConkey agar No. 3 is a selective medium primarily for the isolation of Enterobacteriacae from waters and sewage.

This media differs from the original MacConkey formulation in that as well as bile salts, crystal violet has been included as an additional selective agent.

This has the effect of inhibiting Gram-positive organisms.

The peptone acts as a nitrogen, carbon and vitamin source. Sodium chloride maintains osmotic balance. Lactose is a carbohydrate and during its fermentation causes a confined pH drop around the bacterial colony. This causes a colour change in the pH indicator, neutral red and bile precipitation.

Used for the isolation of Enterobacteriacae from waters and sewage.

Additional Information

Shelf Life (days): 1095 days

Storage Temp: 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale red with slight violet tinge

Dehydrated PH: 7.1±0.2

Prepared PH: 7.1±0.2

Recommended Incubation: Aerobically at 35-37°C for 18-24 hours

Formula*g/L

Peptone: 20.0

Lactose: 10.0

Bile salts no.3: 1.5

Sodium chloride: 5.0

Neutral red: 0.03

Crystal violet: 0.001

Agar: 15.0

Total: 51.5

*Adjusted/supplemented as required to meet performance requirements

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