Lactose Broth

Lactose broth is used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples. This medium is also frequently used as a pre-enrichment medium when testing foods, water samples and dairy products for Salmonella spp.

Beef extract and gelatin peptone provide the required carbon, nitrogen and vitamins in this medium.

Lactose is a fermentable carbohydrate. Fermentation of lactose is detected by the production of gas

Used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale Straw

Dehydrated PH: 6.9±0.2

Prepared PH: 6.9±0.2

Recommended Incubation: Aerobically at 35±2°C for 24-48 hours

Formula*g/L

Beef extract: 3.0

Gelatin peptone: 5.0

Lactose: 5.0

Total: 13.0

*Adjusted/supplemented as required to meet performance requirements