LB Broth (Miller)

Luria Bertani (LB) broth is used for the cultivation and maintenance of recombinant strains of Escherichia coli for molecular biology procedures.

Enzymatic digest of casein provides the required nitrogen and carbon. Vitamin B complex required by recombinant strains of Escherichia coli are supplied by yeast extract. Sodium chloride maintains the osmotic balance and provides sodium ions for membrane transport.

Used for the cultivation and maintenance of recombinant strains of Escherichia coli for molecular biology procedures.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Straw

Dehydrated PH: 7.0±0.2

Prepared PH: 7.0±0.2

Recommended Incubation: Aerobically at 37±1°C for 18-24 hours

Formula*g/L

Enzymatic digest of casein: 10

Yeast extract: 5

Sodium chloride: 10

Total: 25