LB Agar (Miller)

Luria Bertani (LB) agar (Miller) is used for the cultivation and maintenance of recombinant strains of Escherichia coli and is based on the formulation stated by Miller.

This medium is typically used in molecular biology procedures such as the detection of phage or plasmid transformed bacteria.

LB agar (Miller) is prepared using 10 g/L of sodium chloride and this level varies from that described by Lennox.

Enzymatic digest of casein provides the required nitrogen and carbon for organism growth. Vitamin B complex required by recombinant strains of E. coli are supplied by the yeast extract. Sodium chloride maintains the osmotic balance and provides sodium ions for membrane transport.

Used for the cultivation and maintenance of recombinant strains of Escherichia coli.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Straw

Dehydrated PH: 7.0±0.2

Prepared PH: 7.0±0.2

Recommended Incubation: Aerobically at 35-37°C for 18-24 hours

Formula*g/L

Enzymatic digest of casein: 10.0

Yeast extract: 5.0

Sodium chloride: 10.0

Agar: 15.0

Total: 40.0

*Adjusted/supplemented as required to meet performance requirements