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Kligler Iron Agar Group Picture

Kligler Iron Agar

Code: KM0111-500G
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Product Code Pack Size List Price Qty  
KM0111-500G 500g £83.40
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Pack: 500g

£83.40

Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.

Kligler iron agar is a modification of the original formulation developed by Kligler.

It incorporates the principles of Russells double sugar agar with Kligler s lead acetate agar.

The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.Sodium chloride maintains the osmotic balance.

Used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Red

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Recommended Incubation: Aerobically at 35C 2C for 18-24 hours

Formula*g/L

Peptone: 20.0

Sodium chloride: 5.0

Lactose: 10.0

Glucose: 1.0

Ferric citrate: 0.5

Sodium thiosulphate: 0.3

Phenol red: 0.025

Agar: 12.0

Total: 49.0

*Adjusted/supplemented as required to meet performance requirements

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