Hyacinth ETT Crystals (5-ethyl thiotetrazole)

£190.93 to £1167.33

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Variation ID		Product Code	Pack Size	List Price	Qty
15583	photo_camera	NC-0107-E100.0-001	100g NC-0107-E100.0-001	£190.93
15584	photo_camera	NC-0107-F001.0-001	1Kg NC-0107-F001.0-001	£1167.33

5-Ethylthiotetrazole (ETT) is an efficient activator for use in chemical synthesis of either DNA or RNA.

ETT has excellent performance with respect to coupling times, coupling efficiency, consumption of phosphoramidites and reduction of n-1 impurities. ETT can be used for RNA synthesis with TBDMS, O-Methyl, TOM® or ACE® amidites.

Product Name: 5-Ethylmercaptotetrazole
CAS Number / Name: 89797-68-2, 5-Ethyl-1H-thiotetrazole
Molecular Formula: C3H6N4S
Molecular Weight: 130.2 g/mol
Appearance: White crystalline solid
Appearance in acetonitrile: Clear, no precipitation
Colour Index (APHA) of 0.3 M solution in acetonitrile: ≤ 10
Melting Point: 84 - 89°C
Purity by HPLC at 220 nm: ≥ 99.0%
Water Content (KF Coulometric): < 300 ppm

Preparation (IMPORTANT: USE ONLY ANHYDROUS ACETONITRILE WITH A WATER CONTENT OF ≤20 PPM!!)
Choose a Hyacinth ETTTM activator molarity of either 0.25 M or 0.30 M. Use the chart below to determine the volume of acetonitrile required for the amount of Hyacinth ETTTM you wish to dissolve and then completely dissolve Hyacinth ETTTM with this volume. Heating in a warm water bath may speed up dissolution. Use a syringe and two needles (insert one needle as a vent) for water-free addition of acetonitrile to the 30 mL- and 60 mL-bottles.

Amount of Activator	Vol. of Acetonitrile for 0.25 M Solution	Vol. of Acetonitrile for 0.30 M Solution
1.50 (11.5 mmol)	46 mL	38.3 mL
3.00 (23 mmol)	92 mL	76.7 mL
25.00 (192 mmol)	768 mL	640 mL
50.00 (384 mmol)	1536 mL	1280 mL
1000.00 (7.68 mol)	30.72 L	25.6 L

Application Note
Hyacinth ETTTM (5-Ethylmercapto-1H-tetrazole) is an activator for chemical synthesis of RNA and DNA and can be used as a simple substitute for 1H-Tetrazole, DCI and BTT in all protocols.

Precautions for Safe Handling
Hyacinth ETTTM is not a significant hazard in the quantities provided. However, it may enter the body through inhalation, ingestion, eye or skin contact. Avoid prolonged or repeated exposure. If eye or skin contact occurs, wash affected areas with water for 15 minutes and seek medical advice. Seek medical advice if swallowed.

Cas Number: 89797-68-2

Einecs Number: 618-305-8

UN Number: 3224

Hazard Phrases: H315,H319,H335

Prec Phrases: P261