Hyacinth BMT Crystals (5-Benzylmercaptotetrazole, BTT)

Hyacinth BMT activator (also known as 5-Benzylmercapto-1H-tetrazole or BTT)
demonstrates important advantages in the syntheses of oligonucleotides:

• Coupling efficiencies of 99 % with impeccable quality
• Lower percentage of n-1 sequences
• Dramatic reduction of coupling times to under 3 minutes in RNA syntheses
• Efficient RNA synthesis using 50 % or less TBDMS, TOM® or ACE® monomer
• Excellent batch to batch consistency for reproducible and stable oligo production

Product Name: 5-Benzylmercaptotetrazole
Molecular Formula: C8H8N4S
Molecular Weight: 192.2 g/mol
CAS Number and Name: 21871-47-6, 5-Benzyl-1H-thiotetrazole
Appearance in acetonitrile: Clear, no precipitation
Colour Index (APHA): ≤ 100
Melting Point: 136 – 139°C
Purity by HPLC at 220 nm: ≥ 99.0%
Titration: ≥ 98.8%
Water Content (KF Coulometric): ≤ 500 ppm
Shipping: Ambient
Storage: Store the solutions at room temperature (>15°C) to avoid crystallization

Application Note
Hyacinth BMT  (5-Benzylmercapto-1H-tetrazole) is an activator for chemical synthesis of RNA and DNA and can be used as a simple substitute for 1H-Tetrazole, DCI and ETT in all protocols. In case of RNA synthesis it is compatible with both TBDMS and TOM protected monomers. It improves RNA coupling yields to 99%, requires only 3 minute coupling times, requires 50% less phosphoramidite for each coupling and is easy to handle due to low hygroscopicity

Cas Number: 21871-47-6

Einecs Number: 606-853

Hazard Phrases: H315,H318,H335

Prec Phrases: P261,P280,P302+P352,P305+P351+P338