Fastidious Anaerobe Broth

This medium is for the growth of fastidious anaerobes, particularly Bacteroides spp. Fastidious anaerobe broth is also suitable for anaerobic blood culture.

The peptone and yeast extract provides the required carbon, nitrogen and vitamins. Haemin, vitamin k and L-cysteine HCl are growth factors required by some anaerobes. Sodium thioglycollate and L-cysteine HCl reduce the Eh of the medium and the agar helps maintain the Eh. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.

NB: For best results it is recommended that the medium be heated in a boiling water bath, with the cap loosened, and then allowed to cool, with the cap tightened, immediately before use. The cap must be replaced on the container immediately after inoculation.

For the growth of fastidious anaerobes, particularly Bacteroides spp.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Dark Straw

Dehydrated PH: 7.2±0.2

Prepared PH: 7.2±0.2

Recommended Incubation: Aerobically at 37C 1C for 24-48 hours

Formula*g/L

Peptone: 15.0

Yeast extract: 10.0

Sodium thioglycollate: 0.5

Sodium chloride: 2.5

L-cysteine HCI: 0.5

Resazurin: 0.001

Sodium bicarbonate: 0.4

Haemin: 0.005

Vitamin K: 0.0005

Agar: 0.75

Total: 29.7

*Adjusted/supplemented as required to meet performance requirements