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De Man, Rogosa & Sharpe (MRS) Agar Group Picture

De Man, Rogosa & Sharpe (MRS) Agar

Code: KM0080-500G
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Product Code Pack Size List Price Qty  
KM0080-500G 500g £90.15
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Pack: 500g

£90.15

This medium is used for the cultivation and enumeration of Lactobacillus spp. MRS agar is based on the formulation by de Man, Rogosa and Sharpe.

The peptone, yeast extract and beef extract provides the reuired carbon, nitrogen and vitamin source. Glucose is a fermentable carbohydrate. The magnesium sulphate and manganese sulphate act as growth stimulants. Potassium phosphate is a buffering agent.

Selectivity of the medium is achieved through the use of ammonium citrate and sodium acetate, inhibiting microorganisms such as streptococci and moulds.

The addition of the surfactant Tween 80 is required to facilitate the uptake of nutrients from Lactobacillus spp.

Used for the cultivation and enumeration of Lactobacillus spp.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Amber

Dehydrated PH: 5.7±0.2

Prepared PH: 5.7±0.2

Recommended Incubation: Aerobically at 30C 1C for up to 3 days

Formula*g/L

Peptone: 10.0

Yeast extract: 4.0

Beef extract: 8.0

Glucose: 20.0

Potassium phosphate: 2.0

Sodium acetate: 4.15

Ammonium citrate: 2.0

Magnesium sulphate: 0.2

Manganese sulphate: 0.05

Agar: 10.0

Total: 60.4

*Adjusted/supplemented as required to meet performance requirements

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