Clostridium Difficile Agar

Clostridium difficile agar, when supplemented, is used for the isolation of C. difficile from samples. This formulation is a modification of CCFA (Cycloserine-Cefoxitin-Fructose agar) developed by George et al.

The proteose peptone act as carbon, nitrogen and vitamin source in this medium. Fructose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Disodium hydrogen phosphate and potassium dihydrogen phosphate are buffering agents. The magnesium sulfate acts as a source of inorganic ions and the agar is the solidifying agent.

This media requires the addition of defibrinated horse blood and Clostridium difficile selective supplement (LS0022).

Used for the isolation of C. difficile from samples.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Straw

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Recommended Incubation: Aerobically at 37C 1C for 24-48 hours

FORMULA*g/L

Proteose peptone: 40

Fructose: 6

Disodium hydrogen phosphate: 5

Potassium dihydrogen phosphate: 1

Magnesium sulfate: 0.1

Sodium chloride: 2

Agar: 14.9

Total: 69