Chromogenic Coliform Agar (CCA)

Code: KM0056-500G

Variation ID		Product Code	Pack Size	List Price	Qty
17977	photo_camera	KM0056-500G	500g KM0056-500G	£411.60

Pack: 500g

£411.60

Chromogenic coliform agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique.

The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide.

Salmon-GAL is used for the detection of -D-galactosidase enzymatic activity.

X-glucuronide is used for the detection of -D-glucoronidase enzymatic activity.?-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies.

Unlike other coliforms, Escherichia coli cleaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colonies.

Tryptophan is used to increase detection reliability by improving the indole reaction.

The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms.

Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance.

The selectivity is attained by the addition of Tergitol 7 as it inhibits the growth of Gram-positive bacteria.

Used for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Prepared Medium Colour: Straw

Dehydrated PH: 6.8±0.2

Prepared PH: 6.8±0.2

Recommended Incubation: Aerobically at 37±1°C for 18-24 hours

Formula*g/L

Enzymatic digest of casein: 1.0

Yeast extract: 2.0