Charcoal Agar

Code: KM0185-500G

Revert

Variation ID	Product Code	Pack Size	List Price	Qty
17976	KM0185-500G	500g	£79.50

Revert

Pack: 500g

£79.50

Charcoal agar is used for the cultivation of fastidious organisms, particularly Bordetella pertussis.

Charcoal agar is prepared according to the formulation developed by Mishulow, Sharpe and Cohen.

This medium is an efficient substitute for Bordet-Gengou agar in the production of B. pertussis vaccines and can be used as a maintenance medium for stock cultures of Bordetella spp.

Beef extract and peptone provide the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance. Starch and charcoal help in absorbing toxic metabolites that are produced during growth of the organism. Nicotinic acid is an essential growth factor for the growth of Bordetella spp. The addition of cephalexin (LS0018) inhibits accompanying contamination in the samples.

NB: This is a basic medium only and contains no additional supplement. If cephalexin is added, it should be noted, that although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow.

Used for the cultivation of fastidious organisms, particularly Bordetella pertussis.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Black

Prepared Medium Colour: Black

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Recommended Incubation: Aerobically (with high humidity) at 35-37C for up to 4 days

Formula*g/L