Variation ID | Product Code | Pack Size | List Price | Qty | ||
---|---|---|---|---|---|---|
photo_camera | NC-0806-M500.0-001 | 500ml
NC-0806-M500.0-001 |
£67.59 | |||
photo_camera | NC-0806-N001.2-001 | 1.25L
NC-0806-N001.2-001 |
£85.06 | |||
photo_camera | NC-0806-N002.5-001 | 2.5L
NC-0806-N002.5-001 |
£113.03 |
For each synthesis cycle, up to 1 to 2% of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences.
For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.
Product Name: Capping B1
Description: 60% Acetonitrile and 40% Acetic anhydride, (v/v)
CAS Number: Acetonitrile/75-05-8, Acetic anhydride/108-24-7
Appearance: Clear Solution
Refractive Index: 1,362 – 1,364
Water Content: ≤ 30.0 ppm
Shipping: Ambient
Storage: Ambient
Application Note
Capping reagent for nucleic acid synthesis.
Cas Number: 75-05-8
Einecs Number: 200-835-2
HS Code / Commodity Code: 38249992
UN Number: 2924
Hazard Class: 3
Packing Group: 2
Hazard Phrases: H225,H302+H312,H314,H330,H335
Prec Phrases: P210,P280,P303+P361+P353,P304+P340,P310,P305+P351+P338