Capping B (THF / pyridine / N-methylimidazole; V / V / V = 80 / 10 / 10)

Code: NC-0803-N002.5-001

Variation ID		Product Code	Pack Size	List Price	Qty
15603	photo_camera	NC-0803-N002.5-001	2.5L NC-0803-N002.5-001	£224.90

Pack: 2.5L

£224.90

For each synthesis cycle, up to 1 to 2% of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences.

For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Product Name: Capping B
Description: 80% Tetrahydrofuran, 10% Pyridine and 10% 1-Methylimidazole (v/v/v)
CAS Number: Tetrahydrofuran/109-99-9, 1-Methylimidazole/616-47-7, Pyridine/110-86-1
Appearance: Clear Solution
Refractive Index: 1,427 – 1,430
Water content: ≤ 50.0 ppm
Shipping: Ambient
Storage: Ambient

Application Note
Capping reagent for nucleic acid synthesis.