Capping A, 20% Acetic Anhydride in Acetonitrile (Acetonitrile / Acetic Anhydride = 80 / 20)

Code: NC-0711-N002.5-001

Variation ID		Product Code	Pack Size	List Price	Qty
17068	photo_camera	NC-0711-N002.5-001	2.5L NC-0711-N002.5-001	£175.95

Pack: 2.5L

£175.95

For each synthesis cycle, up to 1 to 2% of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences.

For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Catalog Number: NC-0711

Product Name: Capping A

Description: 80% Acetonitrile and 20% Acetic anhydride (v/v)

CAS Number: Acetonitrile 75-05-8 Acetic anhydride 108-24-7

Quality Assurance Data

Appearance: Clear Solution

Water content ≤ 20.0 ppm