Campylobacter enrichment broth

Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans.

Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen.

Campylobacter Enrichment Broth (Bolton) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton Broth (2X Concentration) is described by the USDA. Enzymatic Digest of Animal Tissue, Lactalbumin Hydrolysate, and Yeast Extract provide nitrogen, carbon, amino acids, and vitamins in Campylobacter Enrichment Broth.

Hemin and Lysed Horse Blood provide essential growth factors. Sodium Chloride maintains the osmotic balance of the medium. Sodium Pyruvate, Sodium Metabisulfite, and Sodium Carbonate increase the aerotolerance of Campylobacter spp. by acting as oxygen scavengers.

The addition of cefoperazone, cycloheximide, trimethoprim, and vancomycin are selective agents for heavily contaminated samples.

Used with antimicrobics for the selective enrichment of Campylobacter spp.