CLED SI Agar

Cystine Lactose Electrolyte Deficient Single Indicator (CLED SI) Agar is based on Mackey and Sandys formulation and is popular for culturing urine specimens in the clinical laboratory.

The reduced number of electrolyte level prevents swarming of Proteus spp.The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate.

The inclusion of bromothymol blue as a pH indicator allows easy differentiation of lactose and non-lactose fermenting organisms.

L-Cystine is also present to benefit those organisms that have a particular cystine requirement.

Used for culturing urine specimens in the clinical laboratory.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Pale Straw

Prepared Medium Colour: Pale Straw

Dehydrated PH: 7.3 0.2

Prepared PH: 7.3 0.2

Recommended Incubation: Aerobically at 35-37C for 18-24 hours

Formula*g/L

Peptone: 8.0

Beef extract: 3.0

Lactose: 10.0

L-Cystine: 0.128

Bromothymol blue: 0.02

Agar: 15.0

Total: 36.2

*Adjusted/supplemented as required to meet performance requirements