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CLED DI Agar Group Picture

CLED DI Agar

Code: KM0005-500G
Revert
Product Code Pack Size List Price Qty  
KM0005-500G 500g £80.85
Revert
Pack: 500g

£80.85

Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci spp.

Cystine Lactose Electrolyte Deficient Double Indicator (CLED DI) is popular for urine culture in the clinical laboratory.

The reduced number of electrolytes prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue and Andrades as indicators allows easydifferentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement.

Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium.

Used to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci spp.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Pale Straw

Prepared Medium Colour: Green / Blue

Dehydrated PH: 7.5±0.2

Prepared PH: 7.2±0.2

Recommended Incubation: Aerobically at 35-37C for 18-24 hours

Formula*g/L

Peptone: 8.0

Beef extract: 3.0

Lactose: 10.0

L-cystine: 0.0128

Anrades Indicator: 0.08

Bromothymol Blue: 0.02

Agar: 15.0

Total: 36.2

*Adjusted/supplemented as required to meet performance requirements

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