Burkholderia Cepacia Agar Base

Code: KM0076-500G

Variation ID		Product Code	Pack Size	List Price	Qty
17971	photo_camera	KM0076-500G	500g KM0076-500G	£88.65

Pack: 500g

£88.65

Burkholderia cepacia agar base is a selective medium for the detection and isolation of Burkholderia cepacia from cystic fibrosis (CF) patients. This is an important opportunistic pathogen in CF patients and can lead to fatal infection in approximately 20% individuals that have been colonised with B. cepacia complex organisms.

This medium is based on the PC medium described by Gilligan et al.

Magnesium sulphate, ammonium sulphate and ferrous ammonium sulphate supports the growth of B. cepacia. Potassium di-hydrogen phosphate and di-sodium hydrogen phosphate are buffering agents, used to maintain the pH the medium. The phenol red is used as a pH indicator.

If the sodium pyruvate in the medium is metabolised by B. cepacia alkaline by-products are produced which raises the pH. This causes the colour of the medium to turn pink/red around sections of heavy growth on the medium. Bile salts and crystal violet are selective agents.

The associated selective supplement for this medium, LS0125, contains ticarcillin and polymyxin B which further improves the selectivity, particularly with the inhibition of Pseudomonas spp.

Used for the detection and isolation of Burkholderia cepacia from cystic fibrosis (CF) patients.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale Yellow

Dehydrated PH: 6.2±0.2

Prepared PH: 6.2±0.2

Recommended Incubation: Aerobically at 35-37C for 24-72 hours

Formula*g/L