Buffered Peptone Water (ISO)

Formulated to ISO 6579, Buffered Peptone Water (BPW) is a pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective enrichment medium.

Enzymatic digest of casein is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Di-sodium hydrogen phosphate and potassium di-hydrogen phosphate act as buffers in the medium.

This nutritious medium is free from inhibitors and is well buffered to maintain pH 7.0 for the incubation period. Sub-lethal injury to Salmonella spp. occurs in many food processes and this pre-enrichment step greatly increases the chance of their recovery, especially if a low number of cells are present in a sample.

Buffered Peptone Water (BPW) is a pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective enrichment medium.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Pale Straw

Prepared Medium Colour: Pale Straw

Dehydrated PH: 7.0 0.2

Prepared PH: 7.0 0.2

Recommended Incubation: Aerobically at 36-38C for 16-20 hours

Formula*g/L

Enzymatic digest of Casein: 10.0

Sodium chloride: 5.0

Di-sodium hydrogen phosphate: 3.5

Potassium di-hydrogen phosphate: 1.5

Total: 20.0

*Adjusted/supplemented as required to meet performance requirements