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Brain Heart Infusion (BHI) Agar Group Picture

Brain Heart Infusion (BHI) Agar

Code: KM0091-500G
  Pack Size List Price Qty  
photo_camera 500g
KM0091-500G
£79.80
Pack: 500g

£79.80

Brain heart infusion agar is very nutritious general-purpose medium and is suitable for the isolation of most micro-organisms including many fastidious organisms.

The formulation is a modification of that from Rosenow and Hayden.

The medium is not recommended for the determination of haemolytic reactions because of the glucose content.

The nitrogen, vitamin and carbon sources are supplied by the Brain-Heart infusion solids and peptone. Glucose serves as the carbohydrate source and sodium chloride aids in maintaining the osmotic balance. A phosphate buffer, disodium hydrogen phosphate, is incorporated to help neutralize any acids produced as a result of glucose utilization and thus maintain viability of the organisms.

NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result may auto-sterilize the culture.

Used for the isolation of most micro-organisms including many fastidious organisms.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Beige

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Recommended Incubation: Aerobically at 37C for 18-24 hour

Formula*g/L

Brain-heart infusion solids: 17.5

Peptone: 10.0

Glucose: 2.0

Sodium chloride: 5.0

Disodium hydrogen phosphate: 2.5

Agar: 12.0

Total: 49.0

*Adjusted/supplemented as required to meet performance requirements

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