Bile Aesculin Agar

Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci).

Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex.

The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci.

The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.

Used for the differentiation and presumptive identification of Group D streptococci (enterococci).

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm opaque gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Straw

Dehydrated PH: 6.6 0.2

Prepared PH: 6.6 0.2

Recommended Incubation: Aerobically at 30C for 24-48 hours

Formula*g/L

Peptone: 14.0

Bile Salts: 15.0

Ferric Ammonium Citrate: 0.5

Esculin: 1.0

Agar: 14.0

Total: 44.5

*Adjusted/supplemented as required to meet performance requirements