AMA (concentrated ammonia / 40% aqueous methylamine; V / V = 50 / 50)

Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion.
For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Fast: Usage of one volume of AMA under sealed conditions at 65 °C for 10 min

Product Name: AMA
Description: 50:50 mixture of Ammonium hydroxide and aqueous Methylamine (v/v)
CAS Number: Ammonium hydroxide solution, 32%/1336-21-6, Methylamine solution, 40 wt.% in water/74-89-5
Appearance (Color): colorless to almost colorless
Appearance (Form): clear liquid
Concentration of NH3 in used ammonium hydroxide solution: 30.0 – 33.0 %
Concentration of Methylamine in used metylamine solution: 39.0 – 42.0 %
Shipping: Ambient
Storage: Ambient

Application
With AMA the cleavage of the oligonucleotide from the support is accomplished in 5 minutes at room temperature. The deprotection step is carried out at 65°C for a further 5 minutes. Deprotection can also be carried out at lower temperatures as shown in Table. In all cases, no base modification has been observed.

Using AMA, the order of hydrolysis of the base protecting groups is the acetyl group on dC, followed by the benzoyl group on dA, and then the iBu groups from dG. The hydrolysis of Ac-dC is almost instantaneous, thereby precluding the unwanted transamination reaction to the side-product N-Me-dC possible with alkylamine deprotection. AMA deprotection should not be used in the presence of sensitive labels such as fluorescein or TAMRA.

Notes: If the AMA reagent was made up, store tightly sealed in a glass bottle in the refrigerator. The reagent does not decompose but routine opening will lose some of the volatile bases. Dispose properly after 4-6 weeks.

Cas Number: 74-89-5

Einecs Number: 200-820-0

HS Code / Commodity Code: 38249996

UN Number: 2924

Hazard Class: 3

Packing Group: 2

Hazard Phrases: H225,H302,H314,H335,H410

Prec Phrases: P261,P280,P210,P301+P312,P303+P351+P338