0.25 M Hyacinth BMT Solution (BMT in anhydrous acetonitrile)

Hyacinth BMT activator (also known as 5-Benzylmercapto-1H-tetrazole or BTT) demonstrates important advantages in the syntheses of oligonucleotides: • Coupling efficiencies of 99% with impeccable quality

• Lower percentage of n-1 sequences
• Dramatic reduction of coupling times to under 3 minutes in RNA syntheses
• Efficient RNA synthesis using 50% or less TBDMS, TOM® or ACE® monomer
• Excellent batch to batch consistency for reproducible and stable oligo production

Product Name: Hyacinth BMT Solution
Description: 5-Benzylmercaptotetrazole (also known as Benzylthiotetrazole, BTT) in anhydrous Acetonitrile; 0.25 Molar solution
CAS Number: Acetonitrile/75-05-8, BMT/21871-47-6
Purity by HPLC at 220 nm: ≥ 98.0 %
Refractive Index: 1,354 – 1,356
Solubility: Clear, no precipitation
Water Content: ≤ 20.0 ppm
Density (g/L): 803.48

Shipping: Ambient
Storage: Ambient. Store the solutions above 16°C to avoid crystallization of the Hyacinth BMT.

Application Note

Hyacinth BMT Solution (5-Benzylmercapto-1H-tetrazole in anhydrous acetonitrile) is a chemical activator for RNA and DNA synthesis and is used as a substitute for 1H-Tetrazole, DCI and ETT. In case of RNA synthesis it is compatible with both TBDMS and TOM protected monomers. It improves RNA coupling yields to 99%, requires only 3 minutes coupling times, requires 50% less phosphoramidite for each coupling and is easy to handle.

HS Code / Commodity Code: 38249992

UN Number: 1993

Hazard Class: 3

Packing Group: 2

Hazard Phrases: H225,H302+H312+H332,H319

Prec Phrases: P210,P280,P303+P312,P303+P361+P353,P304+P340,P312,P305+P351+P338