According to the UK Neqas CPT report published 23rd Nov 2018 regarding the recent submissions for CAP there was unfortunately a significant increase in the number of Rhodanine techniques submitted. It was commented that this technique is not appropriate as it demonstrates free copper deposits only and not the protein bound copper. In the report UK NEQAS CPT directed participants to a reference in the Journal of Clinical Pathology (J ClinPath, 1978,31 784-790) which unequivocally supports the use of orcein and not Rhodanine for the demonstration of Copper Associated Protein.
Atom Scientific offer two types of Orcein which have been scored by UK NEQAS CPT as the following:
|Stain Kit||Score||Result Date|
|Orcein for CAP||9||Nov 18|
|Orcein for HbAg||9||May 19|
UK NEQAS CPT delivers a standardised UKAS accredited programme, providing participants with a proficiency testing method for evaluating laboratory practice to guide and ensure best practice and gold standard results.
Using validated material of consistent quality for a range of challenges, participants can demonstrate their quality and performance to maintain high standards of laboratory practice.
As part of each UK NEQAS CPT assessment, run scores are audited to provide an in-depth feedback to aid in continual improvements within organisations, provide expert help identifying problems and provide access to troubleshooting expertise.
Scores range from 0-10 and are based on a number of criteria.
UK NEQAS CPT describes a score of 9/10 to show excellent appropriate demonstration of the expected staining results, and a high level of quality.
This kit is used for identifying elastic fibers on formalin-fixed, paraffin-embedded tissue sections, recommended for vascular pathology. Elastic Fibres and laminae - Brown Nuclei and background - Shades of Blue.
Acid Alcohol 1%
Methyl Blue Aqueous 1% Solution
This stain kit is used for the detection of Hepatitis B antigen and copper associated protein in tissue sections.
Hep B Surface Antigen - Brown granules
Copper Associated Protein - Dark Purple/Brown
Elastic Fibres - Brown
Background - Pale Pink/Brown
Potassium Permanganate 0.5%
Denatured Ethanol 70%
Sulphuric Acid 3% Solution
Oxalic Acid 1% Solution