Violet Red Bile Glucose Agar

Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose.

As all Enterobacteriaceae ferment glucose, VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP.

Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator.

Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.

Isolation and enumeration of Enterobacteriaceae in food products.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige/light purple

Prepared Medium Colour: Light purple

Dehydrated PH: 7.4 ± 0.2

Prepared PH: 7.4 ± 0.2

Recommended Incubation: Aerobically at 30-35°C for 18-24 hours