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Triple Sugar Iron Agar Group Picture

Triple Sugar Iron Agar

Code: KM0168-500G
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Product Code Pack Size List Price Qty  
KM0168-500G 500g £71.55
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Pack: 500g

£71.55

Triple sugar iron agar is used to differentiate between some of the Enterobacteriaceae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide. Beef extract, yeast extract and peptone provide the required nitrogen, carbon and vitamins.

Lactose, sucrose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains osmotic balance.

Used to differentiate between some of the Enterobacteriaceae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide.


Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Orange/red

Dehydrated PH: 7.4 ± 0.2

Prepared PH: 7.4 ± 0.2

Recommended Incubation: Aerobically at 37°C for 18-24 hours

Formula* g/L

Beef extract: 3.0

Yeast extract: 3.0

Peptone: 20.0

Sodium chloride: 5.0

Lactose: 10.0

Sucrose: 10.0

Glucose: 1.0

Ferric citrate: 0.3

Sodium thiosulphate: 0.3

Phenol red: 0.025

Agar: 12.0

Total: 65.0

*Adjusted/supplement as required to meet performance requirements

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