Selenite Mannitol Broth Base

Selenite mannitol broth is a modification of selenite broth and is for the selective enrichment of Salmonellae spp. from clinical, food and environmental specimens.

Comparisons have shown that mannitol selenite broth is better than other enrichment broths for the isolation of Salmonellae spp. The peptone acts as a nitrogen, carbon and vitamin source.

Mannitol is a fermentable carbohydrate and sodium phosphate is a buffer. The medium is made selective by the addition of sodium biselenite (KM8021). The fermentation of mannitol by Salmonellae spp. is said to correct the alkaline pH swing which can occur during incubation.

Following overnight incubation subculture(s) are usually made onto one or more of the many selective enteric solid media.

Selective enrichment of Salmonellae spp. from clinical, food and environmental specimens.

Shelf Life (days): 1095

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Dehydrated Medium Colour: Straw

Dehydrated PH: 7.1 ± 0.2

Prepared Medium Appearance: Liquid

Prepared Medium Colour: Clear to Pale Orange

Prepared PH: 7.1 ± 0.2

Recommended Incubation: Aerobically at 35-37°C for 18-24 hours

Formula g/L

Peptone: 5.0

Mannitol: 4.0

Sodium Phosphate: 10.0

Total: 19.0