Developed by Leifson, selenite F broth is a medium for the selective enrichment of Salmonella spp. from both clinical and food samples.

The peptone acts as a nitrogen, carbon and vitamin source. Lactose is a fermentable carbohydrate and sodium phosphate is a buffer. The medium is made selective by the addition of sodium biselenite (KM8021).

Following overnight incubation subculture(s) are usually made onto one or more of the many selective enteric solid media.