Sabouraud Dextrose Agar with Chloramphenicol

Sabouraud dextrose agar with chloramphenicol is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1)

The tryptone and meat peptone provide the required nitrogen, carbon and vitamins. The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.

(2)Chloramphenicol is included to increase the selectivity of the media inhibiting a range of Gram-positive and Gram-negative bacteria.References(1) Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.(2) Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohls and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

Selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology.

Shelf Life (days): 1095 days

Storage: Once opened keep the powdered medium closed to avoid hydration and use before the expiry date on the label.

Dehydrated Medium Appearance: Fine Powder

Dehydrated Medium Colour: Straw

Dehydrated PH: 5.6 ± 0.2

Prepared Medium Appearance: Firm Gel

Prepared Medium Colour: Straw

Prepared PH: 5.6 ± 0.2

Formula g/L

Tryptone: 5.0

Meat peptone: 5.0

Dextrose: 40.0

Agar: 15.0

Chloramphenicol: 0.05

Total: 65.0