Sabouraud Dextrose Agar

Sabouraud Dextrose Agar is one of several media available for the isolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud Dextrose Agar is a modification of a medium originally described by Sabouraud.

The tryptone and meat peptone provide the nitrogen and vitamins required for organism growth in Sabouraud Dextrose Agar.

The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.

Higher levels of selectivity can be achieved against bacterial species by the addition of chloramphenicol.NB: This is a basic medium only and contains no additional supplements.

Isolation of yeasts and fungi suitable for use in all areas of mycology.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Straw

Dehydrated PH: 5.6 ± 0.2

Prepared PH: 5.6 ± 0.2

Recommended Incubation: Aerobically at 25-30°C for 2-7 days. Plates may be checked weekly for up to 5 weeks to confirm a specimen is culture-negative.

Formula: g/L

Tryptone: 5.0

Meat Peptone: 5.0

Dextrose: 40.0

Agar: 15.0

Total: 65

Hazard Phrases: H0001