Pseudomonas Agar Base

This is a selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples. Pseudomonas agar base is on Kings A medium which uses magnesium and potassium salts to enhance pigment production.

The gelatine peptone and acid-hydrolysed casein act as nitrogen, vitamins, and carbon sources. Magnesium chloride and potassium sulphate promote the production of pyocyanin. The medium can be made selective for P. aeruginosa by the addition of Pseudomonas CN Supplement (LS0006).

Alternatively, the medium can be made selective for Pseudomonas spp. generally by the addition of Pseudomonas CFC Selective Supplement (LS0026).

The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism.

selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Pale Straw

Dehydrated PH: 7.1 ± 0.2

Prepared PH: 7.1 ± 0.2

Recommended Incubation: Incubate aerobically at 25°C for 36-48 hours

Formula g/L

Acid hydrolysed casein: 10

Gelatin peptone: 16

Potassium sulphate: 10

Magnesium chloride: 1.4

Agar: 11

Total: 48.4