Primary Membrane Lactose Glucuronide Agar (mLGA)

Primary Membrane Lactose Glucuronide Agar (mLGA) is used for the differentiation and enumeration of Escherichia coli and other coliforms through a single membrane filtration technique.

The peptone and yeast extract provide the required nitrogen, carbon and vitamins. Sodium pyruvate protects injured cells enhancing recovery and growth of coliforms. Sodium lauryl sulphate is a selective agent that inhibits Gram-positive organisms.

Lactose is a fermentable carbohydrate and phenol red is a pH indicator. Lactose fermentation will result in yellow colonies. X-glucuronide is a chromogenic substrate which can be cleaved by the enzyme -glucuronidase present in E. coli.

This results in a blue colony, but in combination with lactose fermentation colonies will appear green.NB: Prepared plates stored at 28C may show the formation of surface crystals which will disappear when plates are warmed to >20C

Used for the differentiation and enumeration of Escherichia coli and other coliforms through a single membrane filtration technique.

Shelf Life (days): 1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Red

Dehydrated PH: 7.4 ± 0.2

Prepared PH: 7.4 ± 0.2

Recommended Incubation: Aerobically at 30°C ± 2°C for 4 hours and then 35°C ± 2°C for 14-20 hours and examined for growth at a total incubation of 18-24 hours.

Formula g/L

Peptone: 40.0

Yeast extract: 6.0

Lactose: 30.0

Phenol Red: 0.2

Sodium lauryl sulphate: 1.0

Sodium Pyruvate: 0.5

Agar: 10.0

Total: 88.0

Shelf Life (days)	1095 days
Storage Temp (°C)	10-30°C
Dehydrated Medium Appearance	Fine Powder
Prepared Medium Appearance	Firm Gel
Dehydrated Medium Colour	Beige
Prepared Medium Colour	Red
Dehydrated PH	7.4 ± 0.2
Prepared PH	7.4 ± 0.2
Recommended Incubation	Aerobically at 30°C ± 2°C for 4 hours and then 35°C ± 2°C for 14-20 hours and examined for growth at a total incubation of 18-24 hours.