Nutrient Broth No.2

Nutrient Broth No.2 is used for the cultivation of fastidious pathogens and other microorganisms.

This general use medium, rich in nutrients, allows the growth of bacteria when there is a low level of cells. The medium is particularly suitable as a secondary growth medium for staphylococci to be tested for coagulase production and also be used for sterility testing of aerobic organisms.

Beef extract and peptone provide the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.

Used for the cultivation of fastidious pathogens and other microorganisms. 

Shelf Life (days)
1095 days

Storage Temp (°C)
10-30°C

Dehydrated Medium Appearance
Fine Powder

Prepared Medium Appearance
Liquid

Dehydrated Medium Colour
Straw

Prepared Medium Colour
Straw

Dehydrated PH
7.3 ± 0.2

Prepared PH
7.3 ± 0.2

Recommended Incubation
Aerobically at 35-37°C for 18-24 hours

Formula g/L

Beef Extract: 10.0

Peptone: 10.0

Sodium Chloride: 5.0

Total: 25.0