MacConkey Broth (USP)

A selective medium for the enrichment of Enterobacteriaceae and detecting Escherichia coli in pharmaceutical products.

The inclusion of a bromocresol purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of ox bile helps to suppress the growth of Gram-positive and non-enteric bacterial species.

This formulation complies with the Harmonized USP/EP/JP. Nitrogen is supplied by the gelatin peptone whilst lactose serves as the fermentable carbohydrate source.

Oxbile is the selective agent helping to suppress Gram-positive organisms and bromocresol purple detects the pH change as a result of the fermentation of lactose.

A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products.

Shelf Life (days):1095 days

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Liquid

Dehydrated Medium Colour: Beige

Prepared Medium Colour: Purple

Dehydrated PH: 7.3 ± 0.2

Prepared PH: 7.3 ± 0.2

Recommended Incubation: Aerobically at 42-44°C for 24-48 hours

Formula g/L:

Pancreatic digest of gelatin: 20

Lactose: 10

Oxbile: 5

Bromocresol purple: 0.01

Total: 35