QMS ISO 9001:2015
ISO 15189 Compliant
A triple stain pack for the rapid differential evaluation of blood smears, and is directly comparable to diff-quick (a registered trademark of Harleco)
PRINCIPLE
This triple stain pack is for the rapid differential evaluation of haematological and cytological smears, and is directly comparable to DIFF-QUICK.
SPECIMEN COLLECTION
Fresh uncoagulated venous or arterial blood should be used. If the smear is not prepared immediately, Davidsohn and Henry recommended the use of K3EDTA in a concentration of 1.0 to 2.0 mg/ml of blood as the anticoagulant of choice.
If the smear is not to be prepared immediately, thoroughly mix the blood when it is drawn and again before it is examined. This is best done by rotating the specimen for not less than 2 minutes on a mechanical rotator. During the interval, the blood sample should be kept at room temperature and not to exceed six hours from the time of collection. Do not freeze.
PROTOCOL
1. Prepare thin blood smears on grease free slides, and air dry.
2. Fix by immersion in Solution A for 5 seconds.
3. Transfer, without rinsing or drying, to solution B and stain for 5 seconds by slowly agitating the slide in the solution or immersing and withdrawing the slide several times during a 5-second period. Drain excess stain onto absorbent paper.**
4. Transfer slide to solution C and repeat staining as for solution B. (see Caution note below)
5. Rinse the slide briefly in buffered water (pH 6.8) and allow to dry.
RESULTS
Examine under microscope. Staining results will be similar to Romanowsky stained smears.
NOTES
Staining times may be modified to provide varying intensities of staining.
Staining solution should be stored at room temperature in tightly stoppered bottles. When in use aliquot into coplin or similar staining jars.
DO NOT MIX USED STAIN WITH UNUSED STAIN
Transfer of solution B into solution C will result in the deterioration of staining performance over time due to the interaction between the solutions producing insoluble Methylene Blue/Azure – Eosinates. The amount of solution B transferred should be kept to the minimum. Slides may be briefly rinsed in buffer pH 6.8 before transfer to solution C without affecting staining performance.
If slides are to be stored for a long time it is recommended that the Fixation period (step 1) be lengthened to 1-2 minutes.
Kit contents:
RRSP133 Solution A (Fixative UN1230)
RRSP134 Solution B (Acid Dye)
RRSP135 Solution C (Basic Dye)
Protocol Sheet
Hazard Class: 3
Hazard Phrazes: Highly flammable liquid and vapour. Toxic if swallowed. Toxic if inhaled. Causes damage to organs
Prec Phrazes: Keep away from heat/sparks/open flames/hot surfaces. Do not breathe dust/fume/gas/mist/vapours/spray.
| Code | Pack | Price | Qty | |
|---|---|---|---|---|
| RRSK1-100 | 100 Test | £14.18 | ||
| RRSK1-200 | 200 Test | £18.53 | ||
| RRSK1-500 | 500 Test | £31.97 | ||
| RRSK1-1000 | 1000 Test | £45.78 |
